Antibiotics can effectively inhibit the infection of pathogenic microorganisms and the growth and reproduction of bacteria, so they are used to treat various human and animal diseases. Antibiotics are widely used in agriculture, animal husbandry and aquaculture. In addition to disease prevention and treatment, antibiotics are often added in feed to promote the growth and development of animals. Although the use of antibiotics has increased the production of agriculture, animal husbandry and aquaculture, thus bringing huge economic benefits, the resulting residual problems cannot be ignored. In this review, the research status of various kinds of electrochemical sensors used in the detection of antibiotics in water is reviewed, and the preparation, performance, mechanism, advantages and disadvantages of modified electrodes for electrochemical detection methods are categorically analyzed.