CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome

被引：44

作者：

Li Hao ^{[1
]}

Qin Ruiying ^{[1
]}

Liu Xiaoshuang ^{[1
]}

Liao Shengxiang ^{[1
]}

Xu Rongfang ^{[1
]}

Yang Jianbo ^{[1
]}

Wei Pengcheng ^{[1
]}

机构：

[1] Anhui Acad Agr Sci, Inst Rice Res, Key Lab Rice Genet & Breeding, Hefei 230031, Peoples R China

来源：

RICE SCIENCE | 2019年 / 26卷 / 02期

基金：

中国国家自然科学基金;

关键词：

TARGET BASE; DNA;

D O I：

10.1016/j.rsci.2018.07.002

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A.T to G.C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.

引用

页码：125 / 128

页数：4

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