Kinetic model of double-stranded RNA formation during long RNA replication by Qβ replicase

被引：8

作者：

Usui, Kimihito ^{[1
]}

Ichihashi, Norikazu ^{[1
,2
]}

Kazuta, Yasuaki ^{[1
]}

Matsuura, Tomoaki ^{[1
,3
]}

Yomo, Tetsuya ^{[1
,2
,4
]}

机构：

[1] Japan Sci & Technol Agcy, Exploratory Res Adv Technol, Suita, Osaka, Japan

[2] Osaka Univ, Dept Bioinformat Engn, Grad Sch Informat Sci & Technol, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Grad Sch Engn, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

来源：

FEBS LETTERS | 2013年 / 587卷 / 16期

关键词：

Q beta replicase; Double-stranded RNA; Long RNA replication; Kinetic model; COLIPHAGE-Q-BETA; SELF-ENCODED REPLICASE; GLOBIN MESSENGER-RNA; CELL-FREE SYSTEM; QBETA-REPLICASE; TRANSLATION; AMPLIFICATION; RECOMBINATION; COMPLEMENTARY; INITIATION;

D O I：

10.1016/j.febslet.2013.06.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Q beta replicase is an RNA-dependent RNA polymerase, which synthesizes the complementary RNA using a single-stranded RNA as a template. The formation of non-replicable double-stranded RNA (dsRNA) by hybridization between newly synthesized RNA and the template RNA hinders the broader application of Q beta replicase. Here, we developed a kinetic model of Q beta RNA replication consisting of two reaction pathways of dsRNA formation, which quantitatively explains the dynamics of dsRNA formation of three template RNAs. We also found that part of the Q beta phage genomic RNA sequence including the central hairpin loop significantly decreases the rate of dsRNA formation. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2565 / 2571

页数：7

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