Fluorescence depolarization in a scattering medium: Effect of size parameter of a scatterer

被引：16
|
作者：
Ghosh, N [1 ]
Majumder, SK [1 ]
Gupta, PK [1 ]
机构：
[1] Ctr Adv Technol, Biomed Applicat Sect, Indore 452013, India
来源：
PHYSICAL REVIEW E | 2002年 / 65卷 / 02期
关键词：
D O I：
10.1103/PhysRevE.65.026608
中图分类号：
O35 [流体力学]; O53 [等离子体物理学];
学科分类号：
070204 ; 080103 ; 080704 ;
摘要：
For a monodisperse scattering medium, we investigate the dependence on scatterer size parameter for the change in anisotropy of fluorescence due to single scattering at excitation or emission wavelength. The value for the ratio of the anisotropy of fluorescence after one scattering at excitation or emission wavelength to the initial value was observed to increase with increasing value of scatterer size parameter. The effect of multiple scattering on anisotropy of fluorescence from fluorophores embedded in a scattering medium was incorporated using a photon migration model. The model was validated by experiments carried out on samples with known concentration of polystyrene microspheres as scatterers and riboflavins or reduced form of nicotinamide adenine dinucleotide as fluorophores.
引用
收藏
页码：1 / 026608
页数：6
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