Localization of Spindle Checkpoint Proteins in Cells Undergoing Mitosis With Unreplicated Genomes

CHO cells can be arrested with hydoxyurea at the beginning of the DNA synthesis phase of the cell cycle. Subsequent treatment with the xanthine, caffeine, induces cells to bypass the S-phase checkpoint and enter unscheduled mitosis [Schlegel and Pardec, 1986, Science 232: 1264-1266]. and Pardec, 1986, Science 232:1264-1266]. These treated cells build a normal spindle and distribute kinetochores, unattached to chromosomes, to their daughter cells [Brinkley et al., 1988, Nature 336:251-254: Zinkowski et al., cells [Brinkley et al., 1988, Nature 336:251-254; Zinkowski et al., 1991, J Cell Biol 113:1091-1110; Wise and Brinkley, 1997, Cell Motil Cytoskeleton 36:291-302; Balezon et al., 2003, Chromosoma 112:90-102]. To investigate how these cells distribute kinetochores to daughter cells, we analyzed the spindle checkpoint components, Mad2, CENP-E, and the 3F3 phosphoepitope, using immunofluorescence and digital microscopy. Even though the kinetochores were unpaired and DNA was fragmented, the tension, alignment, and motor components of the checkpoint were found to be present and localized as predicted in prometaphase and metaphase. This unusual mitosis proves that a cell can successfully localize checkpoint proteins and divide even when kinetochores are unpaired and fragmented. Cell Motil. Cytoskeleton 65: 890-895, 2008. (C) 2008 Wiley-Liss, Inc.