Dynamics of water extractable organic carbon at a subtropical catchment using fluorescence excitation-emission matrix spectroscopy coupled with parallel factor analysis

The vertical and horizontal movement of water extractable organic carbon (WEOC) has a significant influence on soil organic carbon (SOC) storage and water quality. However, how the amount, components and chemical qualities of WEOC change at horizontal and vertical levels, as well as desorption in the cascade model in subtropical red soils, are not fully understood. We sampled soils from four types of land (Elaeocarpus, Masson Pine, Crape-Myrtle and a deposition site) in a subtropical catchment as replicated cores (n= 96) and investigated changes in the molecular and structural constituents of WEOC using excitation-emission matrix fluorescence and UV-visible absorbance spectroscopy. Our results showed that WEOC concentration changed remarkably in all four plots, and that the concentration coupled with the relative abundance of aromatic compounds decreased with increasing soil depth, whereas low-molecular-weight materials increased with increasing soil depth. Five fluorescent components were identified by parallel factor analysis (i.e., tryptophan-like, tyrosine-like and three humic-substance-like components). The relative abundance of these components suggested that WEOC composition shifted along vertical soil layers. Dynamic variations in fluorescent indices indicated that WEOC from the subsoil has strong biological characteristics. Additionally, there was no significant correlation between WEOC and SOC in subsoil, indicating that much of the WEOC within mineral soils is of local microbial origin rather than from local soil organic matter. Sorption processes alone or stepwise exchange processes of WEOC on the soil minerals do not readily occur, as the red soil in a subtropical area is typically unsaturated and potentially has a large sorption capacity. Once soil carbon is adsorbed, it is difficult to release it into soil water by microbial processing. Data from the deposition site suggest that components of hydrophilic WEOC with low molecular weight are preferentially transported.