A spectrofluorometric method for microdetermination of H2O2 has been developed. The method is based on the oxidation of hydrogen peroxide with eerie ion in acid solution and measurement of the fluorescence during titration of the Ce(III) ions produced. The fluorescent species have excitation and emission maxims at 260 and 360 nm, respectively. The detection limit of measurement by this method was 0.1 ppm hydrogen peroxide.
机构:
City Univ Hong Kong, Dept Biol & Chem, Tat Chee Ave, Kowloon, Peoples R ChinaCity Univ Hong Kong, Dept Biol & Chem, Tat Chee Ave, Kowloon, Peoples R China
Tanner, PA
Wong, AYS
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机构:
City Univ Hong Kong, Dept Biol & Chem, Tat Chee Ave, Kowloon, Peoples R ChinaCity Univ Hong Kong, Dept Biol & Chem, Tat Chee Ave, Kowloon, Peoples R China