Developmental sex differences in glutamic acid decarboxylase (GAD65) and the housekeeping gene, GAPDH

被引：39

作者：

Perrot-Sinal, TS

Davis, AM

McCarthy, MM

机构：

[1] Univ Maryland, Sch Med, Dept Physiol, Baltimore, MD 21201 USA

[2] Eunice Kennedy Shriver Ctr Mental Retardat Inc, Dept Biomed Sci, Waltham, MA 02452 USA

来源：

BRAIN RESEARCH | 2001年 / 922卷 / 02期

关键词：

GABA system; sex difference; sexual differentiation;

D O I：

10.1016/S0006-8993(01)03167-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Previous work has demonstrated that the GABAergic system is involved in sexual differentiation of the rodent hypothalamus. The present study was designed to further examine this involvement by investigating developmental sex differences in GAD(65) protein levels in hypothalamic and extrahypothalamic brain regions known to be sexually dimorphic in adulthood. Brain nuclei were micro-dissected and GAD(65) protein levels were quantified using western immunoblotting. Sex differences in levels of GAD(65) were found in the dorsomedial nucleus and preoptic area of the hypothalamus and also the medial amygdaloid nucleus and CA1 subfield of the hippocampus. Unexpectedly, there were sex differences in protein levels of the housekeeping gene, GAPDH, cautioning against the use of GAPDH for standardizing protein samples during western immunoblotting. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：201 / 208

页数：8

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