HLA Evolutionary Divergence as a Prognostic Marker for AML Patients Undergoing Allogeneic Stem Cell Transplantation

The diversity of human leukocyte antigens (HLAs) enables the presentation of immense repertoires of peptides, including tumor-associated antigens (TAAs). As a surrogate for immunopeptidome diversity, the HLA evolutionary divergence (HED) between individual HLA alleles might directly define the ability to present TAAs, a prerequisite for graft-versus-leukemia effects. We therefore analyzed the impact of HED on survival within a cohort of 171 acute myeloid leukemia (AML) patients after matched donor allogeneic hematopoietic stem cell transplantation (HSCT). Low HED (<25th percentile) of HLA class I (HEDclass I) or HLA-DR antigens (HEDDR) was a strong determinant for adverse overall survival after allogeneic HSCT (OS), with a hazard ratio for death of 1.9 (95% CI 1.2-3.2) and 2.1 (95% CI 1.3-3.4), respectively. Defining a cutoff value for the combined HEDtotal(HED(class I)and HEDDR), the respective 5 year OS was 29.7% and 64.9% in patients with low and high HEDtotal(p< 0.001), respectively. Furthermore, the risk of relapse was significantly higher in patients with low HEDtotal(hazard ratio (HR) 2.2, 95% CI 1.3-3.6) and event-free survival (EFS) was significantly reduced (5 year EFS 25.7% versus 54.4%,p< 0.001). We here introduce HED, a fundamental metric of immunopeptidome diversity, as a novel prognostic factor for AML patients undergoing allogeneic HSCT.