Evaluation of PVA hydrogel as an extracellular matrix for in vitro study of fibroblast proliferation

被引:5
|
作者
Sankari, Luciana [1 ]
Fernandes, Beatriz L. [2 ]
Rebelatto, Carmen L. K. [3 ]
Brofman, Paulo R. S. [3 ]
机构
[1] Univ Posit, Grad Estet & Cosm, Curitiba, Parana, Brazil
[2] Univ Catolica Parana, Programa Posgrad Tecnol Saude, Curitiba, Parana, Brazil
[3] Pontificia Univ Catolica Parana, Ctr Tecnol Celular, Curitiba, Parana, Brazil
关键词
Cytotoxicity assay; In vitro cells proliferation; PVA extracellular matrix; PVA hydrogel; CELL; RECEPTOR; ASSAY;
D O I
10.1080/00914037.2019.1596915
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Synthetic 3 D extracellular matrix (ECM) have been used to evaluate cell behavior in vitro. However, how cells and the growth factors interact with the ECM is still not fully understood. The objective of the present work is to evaluate in vitro L929 mouse fibroblasts in contact with Poly(vinyl alcohol) (PVA) hydrogel added with the growth factors: fibroblast (FGF), transforming-beta 1 (TGF-beta 1), vascular endothelial (VEGF), insulin-like (IGF), and epidermal (EGF). The cytotoxicity assay showed that the maximum concentration of PVA hydrogel per well must be 10 vol%. When the factors EGF and FGF were added to the wells individually, it was possible to increase the concentration of PVA hydrogel to 15.04% and 18.81%, respectively, without interfering with the level of cytotoxicity. The IGF and the TGF-beta 1, when individually incorporated into the PVA hydrogel, affected negatively the cell viability (7.04% and 7.89%, respectively) compared to the other. The smallest allowable concentration occurred when all the factors were incorporated together with the PVA hydrogel (3,6%). Statistical significance (p < 0.05) was obtained between PVA + FGF, PVA + TGF, and PVA + EGF and their controls.
引用
收藏
页码:653 / 658
页数:6
相关论文
共 50 条
  • [31] Partially Digested Adult Cardiac Extracellular Matrix Promotes Cardiomyocyte Proliferation In Vitro
    Williams, Corin
    Sullivan, Kelly
    Black, Lauren D., III
    ADVANCED HEALTHCARE MATERIALS, 2015, 4 (10) : 1545 - 1554
  • [32] Biocompatibility evaluation of dental metal alloys in vitro:: Expression of extracellular matrix molecules and its relationship to cell proliferation rates
    Grill, V
    Sandrucci, MA
    Di Lenarda, R
    Cadenaro, M
    Narducci, P
    Bareggi, R
    Martelli, AM
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 2000, 52 (03): : 479 - 487
  • [33] Evaluation of chitosan/PVA blended hydrogel membranes
    Yang, JM
    Su, WY
    Leu, TL
    Yang, MC
    JOURNAL OF MEMBRANE SCIENCE, 2004, 236 (01) : 39 - 51
  • [34] Preparation and characterization of bovine dental pulp-derived extracellular matrix hydrogel for regenerative endodontic applications: an in vitro study
    Elnawam, Hisham
    Thabet, Abdelrahman
    Mobarak, Ahmed
    Abdallah, Amr
    Elbackly, Rania
    BMC ORAL HEALTH, 2024, 24 (01):
  • [35] Study on PVA hydrogel crosslinked by epichlorohydrin
    Bo, Jiang
    Journal of Applied Polymer Science, 1992, 46 (05): : 783 - 786
  • [36] Hydrogel scaffolds as in vitro models to study fibroblast activation in wound healing and disease
    Smithmyer, Megan E.
    Sawicki, Lisa A.
    Kloxin, April M.
    BIOMATERIALS SCIENCE, 2014, 2 (05) : 634 - 650
  • [37] Nerve Guidance by a Decellularized Fibroblast Extracellular Matrix
    Harris, Greg M.
    Madigan, Nicolas N.
    Lancaster, Karen Z.
    Enquist, Lynn W.
    Windebank, Anthony J.
    Schwartz, Jeffrey
    Schwarzbauer, Jean E.
    MATRIX BIOLOGY, 2017, 60-61 : 176 - 189
  • [38] Structural biology response of a collagen hydrogel synthetic extracellular matrix with embedded human fibroblast: computational and experimental analysis
    Manzano, Sara
    Moreno-Loshuertos, Raquel
    Doblare, Manuel
    Ochoa, Ignacio
    Doweidar, Mohamed H.
    MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, 2015, 53 (08) : 721 - 735
  • [39] Structural biology response of a collagen hydrogel synthetic extracellular matrix with embedded human fibroblast: computational and experimental analysis
    Sara Manzano
    Raquel Moreno-Loshuertos
    Manuel Doblaré
    Ignacio Ochoa
    Mohamed Hamdy Doweidar
    Medical & Biological Engineering & Computing, 2015, 53 : 721 - 735
  • [40] Proliferation of the human periodontal ligament fibroblast by laser biostimulation: An in vitro study
    Shelly, Ahuja
    Shaila, Kothiwale
    Kishore, Bhat
    MECHANISMS FOR LOW LIGHT THERAPY, 2006, 6140