Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP

被引：7

作者：

Zhao, Min ^{[1
]}

Yang, Lingye ^{[1
]}

Zhang, Ruichen ^{[1
]}

Dong, Jie ^{[1
]}

Dong, Haifeng ^{[2
]}

Wen, Yongqiang ^{[2
]}

Zhan, Xiaowei ^{[3
]}

Wang, Ge ^{[1
]}

Lu, YunFeng ^{[1
]}

Wang, Guojie ^{[1
]}

机构：

[1] Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China

[2] Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China

[3] Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China

来源：

POLYMER | 2013年 / 54卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Hybridization; Fluorescence; Polyelectrolytes; CATIONIC CONJUGATED POLYMERS; CALF THYMUS DNA; ENERGY-TRANSFER; NUCLEIC-ACIDS; HYDROPHOBIC INTERACTIONS; SEQUENCE DETECTION; BINDING-PROPERTIES; MOLECULAR BEACONS; PROBES; RNA;

D O I：

10.1016/j.polymer.2012.11.029

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A novel pyrene-labeled polyelectrolyte (Py-PDMAEMA(+)) has been prepared by atom transfer radical polymerization (ATRP) for detection of DNA hybridization. The electrostatic and hydrophobic interactions between Py-PDMAEMA(+) and ssDNA (hairpin or linear DNA) kept them forming a complex probe which could be used as a turn-off fluorescent sensor. The fluorescence intensity of the probe decreased upon adding the complementary strand, since the pyrene moiety could be intercalated into the duplex and thus the fluorescence quenching by nucleotide bases was strengthened. The proposed intercalation mode was confirmed by the circular dichroism spectra and the fluorescence quenching study with iodide. This pyrene-labeled polyelectrolyte combined with ssDNA may establish a novel fluorescence sensing system for DNA hybridization. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：297 / 302

页数：6

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