The Linghu Au deposit is located within the northeastern part of the Xiaoqinling region in the southern margin of the North China Craton. Field investigations, cross-cutting relationships, and mineral paragenetic associations have identified three stages of hydrothermal activity. To determine the origin and evolution of ore-forming fluids, and better understand genesis of the Linghu Au deposit, we have undertaken a series of studies including fluid inclusion and He-Ar-H-O-S-Pb isotope systematics. Fluid inclusion study indicates that the early stage of gold mineralization was characterized by a high-temperature and low-salinity H2O-CO2-NaCl hydrothermal fluids that have delta D values of - 82.0 to - 78.6 parts per thousand and delta O-18(H2O) values of 5.3 to 6.4 parts per thousand. The intermediate stage of gold mineralization involved moderate temperature H2O-CO2-NaCl hydrothermal fluids with delta D and delta O-18(H2O) values of - 92.7 parts per thousand to - 87.9 parts per thousand and - 1.4 parts per thousand to 0 parts per thousand, respectively. Pyrite samples from this mineralization stage have He-3/He-4 and Ar-40/Ar-36 ratios of 0.26-0.31 Ra and 2834.7-4300.6, respectively. The last mineralization stage involved (low-temperature and low-salinity NaCl-H2O system of hydrothermal fluids with relatively lower delta D values of - 93.1 to - 92.3 parts per thousand and delta O-18(H2O) values of - 5.7 to - 5.2 parts per thousand. Moreover, sulfides in ores yielded delta S-34 values of - 3.6 to 5.9 parts per thousand, with an average of 2.65 parts per thousand. The sulfides have Pb-206/Pb-204 ratios of 17.101-17.804, Pb-207/Pb-204 ratios of 15.438-15.554, and Pb-208/Pb-204 ratios of 37.438-38.654. The results of fluid inclusions and H-O, S-Pb, and He-Ar isotopes indicate that hydrothermal fluids associated with the early stage of mineralization originated from magmatic fluids, with sulfur and lead being derived from both units of the Taihua Group and a Late Triassic magmatic-hydrothermal system. All of these data indicate that the Linghu gold deposit formed as a result of magmatic-hydrothermal mineralizing processes.