Strategies to improve the surface plasmon resonance-based immmunodetection of bacterial cells

被引：7

作者：

Charlermroj, Ratthaphol ^{[1
,2
]}

Oplatowska, Michalina ^{[2
]}

Gajanandana, Oraprapai ^{[1
]}

Himananto, Orawan ^{[1
]}

Grant, Irene R. ^{[2
]}

Karoonuthaisiri, Nitsara ^{[1
,2
]}

Elliott, Christopher T. ^{[2
]}

机构：

[1] NSTDA, Natl Ctr Genet Engn & Biotechnol BIOTEC, Pathum Thani 12120, Thailand

[2] Queens Univ Belfast, Sch Biol Sci, Inst Global Food Secur, Belfast BT9 5AG, Antrim, North Ireland

来源：

MICROCHIMICA ACTA | 2013年 / 180卷 / 7-8期

关键词：

Immunosensor; Seedborne bacterial pathogens; Surface plasmon resonance; MONOCLONAL-ANTIBODY; BIOSENSOR; ENHANCEMENT; SENSITIVITY; SALMONELLA; CITRULLI;

D O I：

10.1007/s00604-013-0975-x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have made a comparison of (a) different surface chemistries of SPR sensor chips (such as carboxymethylated dextran and carboxymethylated C1) and (b) of different assay formats (direct, sandwich and subtractive immunoassay) in order to improve the sensitivity of the determination of the model bacteria Acidovorax avenae subsp. citrulli (Aac). The use of the carboxymethylated sensor chip C1 resulted in a better sensitivity than that of carboxymethylated dextran CM5 in all the assay formats. The direct assay format, in turn, exhibits the best sensitivity. Thus, the combination of a carboxymethylated sensor chip C1 with the direct assay format resulted in the highest sensitivity for Aac, with a limit of detection of 1.6 x 10(6) CFU mL(-1). This SPR immunosensor was applied to the detection of Aac in watermelon leaf extracts spiked with the bacteria, and the lower LOD is 2.2 x 10(7) CFU mL(-1).

引用

页码：643 / 650

页数：8

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