Quantification of Somatostatin with a Surface Plasmon Resonance-based Biosensor

被引：0
|
作者：
Gao Jin-Hang [1 ]
Liu Rui [1 ]
Tong Huan [2 ]
Li Xian [1 ]
Qang Ou [1 ]
Tang Cheng-Wei [1 ,2 ]
机构：
[1] Sichuan Univ, W China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China
[2] Sichuan Univ, W China Hosp, Dept Gastroenterol, Chengdu 610041, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2010年 / 31卷 / 07期
关键词：
Quantification of somatostatin; Surface plasmon resonance; Antigen-antibody-antigen binding assay; IMMUNOSENSOR; ANTIGEN;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A quick technique of quantifying somatostatin (SST) with surface plasmon resonance (SPR) on the principle of antigen-antibody-antigen assay was established. The optimal coupling of CM5 sensor chip for SST was indicated as 1231.4 response unit (RU) at pH = 5.0, and the SST concentration of 75 mu g/mL. A negative linear relationship between SST content (20-2000 pg) and RU was obtained using the high molecular mass antibody for SST at 117.7 nmol/L, R = -0.824, p<0.05, coefficient of variation 1.3%. This method can be used to detect SST content at a range from 20 to 2000 pg with good repetition and real-time, which would be useful for clinical diagnosis and intensive care of critically ill patients.
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收藏
页码：1327 / 1331
页数：5
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