Comparative study of conductometric glucose biosensor based on gold and on magnetic nanoparticles

被引：28
|
作者：
Nouira, Wided [1 ,2 ]
Maaref, Abderrazak
Elaissari, Hamid [3 ]
Vocanson, Francis [4 ]
Siadat, Maryam [5 ]
Jaffrezic-Renault, Nicole [1 ]
机构：
[1] Univ Lyon 1, Inst Sci Analyt, F-69622 Villeurbanne, France
[2] Univ Monastir, Lab Interfaces & Mat Avances, Fac Sci Monastir, Monastir 5019, Tunisia
[3] Univ Lyon 1, Lab LAGEP CPE 308G, F-69622 Villeurbanne, France
[4] Univ St Etienne, Lab Hubert Curien, Univ Lyon, F-42023 St Etienne, France
[5] Univ Metz, LASC, ISEA, F-57070 Metz, France
来源：
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2013年 / 33卷 / 01期
关键词：
Magnetic nanoparticles; Gold nanoparticles; Conductometric biosensor; Polyelectrolyte; Glucose oxidase; CARBON NANOTUBES; DNA HYBRIDIZATION; AU ELECTRODE; ENZYME; OXIDASE; IMMOBILIZATION; SURFACE; SENSOR; UREA; NANOTECHNOLOGY;
D O I：
10.1016/j.msec.2012.08.043
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The aim of this study was to show the feasibility and the performances of nanoparticle biosensing. A glucose conductometric biosensor was developed using two types of nanoparticles (gold and magnetic), glucose oxidase (GOD) being adsorbed on PAH (poly(allylamine hydrochloride)) modified nanoparticles, deposited on a planar interdigitated electrode (IDES). The best sensitivities for glucose detection were obtained with magnetic nanoparticles (70 mu M/mM and 3 mu M of detection limit) compared to 45 mu M/mM and 9 mu M with gold nanoparticles and 30 mu M/mM and 50 mu M with GOD directly cross-linked on IDEs. When stored in phosphate buffer (20 mM, pH 7.3) at 4 degrees C, the biosensor showed good stability for more than 12 days. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：298 / 303
页数：6
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