Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato

被引：125
|
作者：
Chun, Se-Chul [1 ]
Chandrasekaran, Murugesan [2 ]
机构：
[1] Konkuk Univ, Dept Bioresource & Food Sci, Seoul 05029, South Korea
[2] Sejong Univ, Dept Food Sci & Biotechnol, 209 Neungdong Ro, Seoul 05006, South Korea
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 125卷
关键词：
Chitosan; Chitosan nanoparticles; Chitinase; beta-1,3-Glucanase; Pathogenesis-related proteins; Fusarium andiyazi; Antioxidant enzymes; VITRO ANTIFUNGAL EFFICACY; BIOLOGICAL-CONTROL; PLANT; RESISTANCE; FRUIT; OLIGOCHITOSAN; MECHANISMS; PROTECTION; INDUCTION; IMMUNITY;
D O I：
10.1016/j.ijbiomac.2018.12.167
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this work was to evaluate the possibility of control of wilt disease caused by Fusarium andiyazi through chitosan (CS) and chitosan nanoparticles (CNPs). In the present study, the expression pattern of pathogenesis-related (PR) proteins genes such as PR-1, PR-2 ((beta-1,3-glucanase), PR-8 (chitinase), and PR-10 was analyzed using real-time RT-PCR. In vitro studies showed that among different concentrations (0.1-5.0 mg/ml), 5.0 mg/ml concentration of CS and CNPs produced maximum inhibition of radial mycelial growth, 54.8% and 73.81%, respectively. Also, upregulated expression of beta-1,3-glucanase, chitinase, PR-1 and PR-10 genes were recorded with 1.48, 1.15, 1.15, and 1.41, fold expression in 24 hpi, respectively, in plants inoculated with CNPs. The most significant up-regulation was observed in transcript profile of SOD that showed 4.5-foldexpression, at 48 hpi. Therefore, our results confirmed that CS and CNPs induced up-regulation of PR-proteins and antioxidant genes might play a significant role for successful biocontrol. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：948 / 954
页数：7
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