Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus during freeze-drying

被引:5
|
作者
Qin, Tao [1 ]
Ma, Qi [1 ]
Chen, He [2 ]
Shu, Guowei [1 ,2 ]
机构
[1] Shaanxi Acad Sci, Enzyme Engn Inst, Xian, Peoples R China
[2] Shaanxi Univ Sci & Technol, Coll Life Sci & Engn, Xian, Peoples R China
关键词
Lactobacillus acidophilus; freeze-drying; carbohydrate; cryoprotectant; viable cells; DOUBLE-BLIND; DIARRHEA;
D O I
10.4028/www.scientific.net/AMR.700.259
中图分类号
TU [建筑科学];
学科分类号
0813 ;
摘要
Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus was studied by counting viable cells before and after freeze-drying. The concentrations of five carbohydrates were all 3%, 6%, 9%, 12% and 15%. Results showed as follows: the optimal concentrations of trehalose, soluble starch, raffinose and galactose were 12%, 9%, 3% and 15%, the survival of Lactobacillus acidophilus was 42.62%, 56.50%, 45.05% and 42.69% and the viable cells of lyophilized powder were 0.40x10(11)cfu/g,0.45x10(11)cfu/g, 0.52x10(11) and 1.09x10(11)cfu/g, respectively.
引用
收藏
页码:259 / +
页数:2
相关论文
共 38 条
  • [1] Effect of sucrose, lactose, glucose, maltose on survival of Lactobacillus acidophilus during freeze-drying
    Chen, He
    Ma, Zhenxing
    Shu, Guowei
    Qin, Tao
    Cai Ninggang
    [J]. ADVANCED RESEARCH ON ADVANCED STRUCTURE, MATERIALS AND ENGINEERING, 2012, 382 : 396 - +
  • [2] Effects of Trehalose,Glycerin and NaCl on the Growth and Freeze-drying of Lactobacillus acidophilus
    Chen He
    Wang Lei
    Ji Zhe
    Wang Changfeng
    Shu Guowei
    [J]. ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 2, 2011, : 183 - 185
  • [3] Effects of Trehalose, Glycerin and NaCl on the Growth and Freeze-Drying of Lactobacillus Acidophilus
    Chen He
    Ji Zhe
    Wang Lei
    Shu Guowei
    [J]. INFORMATION TECHNOLOGY AND AGRICULTURAL ENGINEERING, 2012, 134 : 967 - 971
  • [4] Effect of cryoprotective agents on survival and β-galactosidase activity of Lactobacillus acidophilus and Streptococcus thermophilus during freeze-drying
    Selwal, Krishan Kumar
    Selwal, Manjit K.
    Gandhi, D. N.
    [J]. MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, 2011, 66 (01): : 25 - 28
  • [5] Improvement of Freeze-drying Survival Rate of Lactobacillus acidophilus by Cold Shock Treatmen
    Wang, Xiaomeng
    Zhen, Ni
    Tian, Qiyuan
    Ye, Congyan
    Zeng, Xiaoqun
    Pan, Daodong
    Chen, Xiaohu
    [J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21 (03) : 203 - 209
  • [6] Effect of four materials including glycine, arginine, proline and sodium glutamate on survival of Bifidobacterium bifidum during freeze-drying
    Ma, Qi
    Qin, Tao
    Chen, He
    Shu, Guowei
    [J]. ADVANCED RESEARCH ON MATERIAL ENGINEERING AND ELECTRICAL ENGINEERING, 2013, 676 : 93 - +
  • [7] Effects of heat shock treatment on the survival rate of Lactobacillus acidophilus after freeze-drying
    Zhen, Ni
    Zeng, Xiaoqun
    Wang, Huijun
    Yu, Jing
    Pan, Daodong
    Wu, Zhen
    Guo, Yuxing
    [J]. FOOD RESEARCH INTERNATIONAL, 2020, 136
  • [8] Influence of trehalose and moisture content on survival of Lactobacillus salivarius subjected to freeze-drying and storage
    Zayed, G
    Roos, YH
    [J]. PROCESS BIOCHEMISTRY, 2004, 39 (09) : 1081 - 1086
  • [9] Effect of five materials including sucrose, lactose, skim milk, yeast, vitamin B2 on survival of Lactobacillus bulgaricus during freeze-drying
    Chen, He
    Zhang, Qiuhong
    Luo, Qian
    Shu, Guowei
    [J]. ADVANCED RESEARCH ON STRUCTURE, MATERIALS AND ENGINEERING II, 2013, 700 : 255 - 258
  • [10] Control of cell morphology of Lactobacillus acidophilus for enhanced cell stability during freeze-drying and storage
    Senz, M.
    van Lengerich, B.
    Stahl, U.
    Bader, J.
    [J]. NEW BIOTECHNOLOGY, 2012, 29 : S208 - S208