THROMBIN IMMOBILIZATION TO METHACRYLIC ACID GRAFTED POLY(3-HYDROXYBUTYRATE) AND ITS IN VITRO APPLICATION

被引：3

作者：

Akkaya, Alper ^{[1
]}

Pazarlioglu, Nurdan ^{[1
]}

机构：

[1] Ege Univ, Dept Biochem, Fac Sci, TR-35100 Bornova, Turkey

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2013年 / 43卷 / 01期

关键词：

bleeding; graft polymerization; immobilization; methacrylic acid; poly(3-hydroxybutyrate); thrombin; ULTRAFILTRATION MEMBRANES; POLYHYDROXYALKANOATES; COPOLYMERS; POLYMERS;

D O I：

10.1080/10826068.2012.693900

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential support for long-term implants. For this reason, it is a good support for enzyme immobilization. Enzyme immobilization could not be done directly because poly(3-hydroxybutyrate) has no functional groups. Therefore, modification should be done for enzyme immobilization. In this study, methacrylic acid was graft polymerized to poly(3-hydroxybutyrate) and thrombin was immobilized to polymethacrylic acid grafted poly(3-hydroxybutyrate). In fact, graft polymerization of methacrylic acid to poly(3-hydroxybutyrate) and thrombin immobilization was a model study. Biomolecule immobilized poly(3-hydroxybutyrate) could be used as an implant. Thrombin was selected as a biomolecule for this model study and it was immobilized to methacrylic acid grafted poly(3-hydroxybutyrate). Then the developed product was used to stop bleeding.

引用

页码：48 / 59

页数：12

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