THROMBIN IMMOBILIZATION TO METHACRYLIC ACID GRAFTED POLY(3-HYDROXYBUTYRATE) AND ITS IN VITRO APPLICATION

被引：3
|
作者：
Akkaya, Alper [1 ]
Pazarlioglu, Nurdan [1 ]
机构：
[1] Ege Univ, Dept Biochem, Fac Sci, TR-35100 Bornova, Turkey
来源：
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2013年 / 43卷 / 01期
关键词：
bleeding; graft polymerization; immobilization; methacrylic acid; poly(3-hydroxybutyrate); thrombin; ULTRAFILTRATION MEMBRANES; POLYHYDROXYALKANOATES; COPOLYMERS; POLYMERS;
D O I：
10.1080/10826068.2012.693900
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential support for long-term implants. For this reason, it is a good support for enzyme immobilization. Enzyme immobilization could not be done directly because poly(3-hydroxybutyrate) has no functional groups. Therefore, modification should be done for enzyme immobilization. In this study, methacrylic acid was graft polymerized to poly(3-hydroxybutyrate) and thrombin was immobilized to polymethacrylic acid grafted poly(3-hydroxybutyrate). In fact, graft polymerization of methacrylic acid to poly(3-hydroxybutyrate) and thrombin immobilization was a model study. Biomolecule immobilized poly(3-hydroxybutyrate) could be used as an implant. Thrombin was selected as a biomolecule for this model study and it was immobilized to methacrylic acid grafted poly(3-hydroxybutyrate). Then the developed product was used to stop bleeding.
引用
收藏
页码：48 / 59
页数：12
相关论文
共 50 条
  • [1] Microfibrillated Cellulose Grafted with Metacrylic Acid as a Modifier in Poly(3-hydroxybutyrate)
    Popa, Marius Stelian
    Frone, Adriana Nicoleta
    Radu, Ionut Cristian
    Stanescu, Paul Octavian
    Trusca, Roxana
    Raditoiu, Valentin
    Nicolae, Cristian Andi
    Gabor, Augusta Raluca
    Panaitescu, Denis Mihaela
    POLYMERS, 2021, 13 (22)
  • [2] Degradability of poly(3-hydroxybutyrate) and its copolymer grafted with styrene by radiation
    Bahari, K
    Mitoma, H
    Enjoji, T
    Yoshii, F
    Makuuchi, K
    POLYMER DEGRADATION AND STABILITY, 1998, 61 (02) : 245 - 252
  • [3] Scaffolds of chitosan grafted onto poly(3-hydroxybutyrate)
    Olayo, Roberto
    Gonzalez-Torres, Maykel
    Rodriguez-Talavera, Rogelio
    Toral-Morales, Rosa
    Vargas-Munos, Susana
    Morales-Corona, Juan
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 251
  • [4] Synthesis of Poly(3-hydroxybutyrate) by Immobilized Poly(3-hydroxybutyrate) Synthase
    Kenji Tajima
    Hatsumi Tannai
    Yasuharu Satoh
    Masanobu Munekata
    Polymer Journal, 2003, 35 : 407 - 410
  • [5] Immobilization influence on the water sorption and diffusion in poly(3-hydroxybutyrate)
    Iordanskii, AL
    Kamaev, PP
    Zaikov, GE
    JOURNAL OF APPLIED POLYMER SCIENCE, 1999, 73 (06) : 981 - 985
  • [6] Synthesis of poly(3-hydroxybutyrate) by immobilized poly(3-hydroxybutyrate) synthase
    Tajima, K
    Tannai, H
    Satoh, Y
    Munekata, M
    POLYMER JOURNAL, 2003, 35 (04) : 407 - 410
  • [7] Cell Surface Display of Poly(3-hydroxybutyrate) Depolymerase and its Application
    Lee, Seung Hwan
    Lee, Sang Yup
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 30 (02) : 244 - 247
  • [8] Poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate)-based biopolymer systems
    Bonartsev A.P.
    Bonartseva G.A.
    Shaitan K.V.
    Kirpichnikov M.P.
    Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2011, 5 (1) : 10 - 21
  • [9] Structure, mechanism and application of vinyl alcohol oligomers grafted onto poly(3-hydroxybutyrate): a proposal
    Gonzalez Torres, Maykel
    Gonzaez Perez, Manuel
    Reyes Cervantes, Eric
    Cerna Cortez, Jorge Raul
    Vargas Munoz, Susana
    Rodriguez Talavera, Jose Rogelio
    E-POLYMERS, 2014, 14 (06): : 397 - 405
  • [10] The vitro and vivo study of Poly (3-hydroxybutyrate) microspheres
    Tian, Feng
    Zhao, Yong-Liang
    Liu, Chang-Jun
    Li, Fan
    Xing, Nan
    APCMBE 2008: 7TH ASIAN-PACIFIC CONFERENCE ON MEDICAL AND BIOLOGICAL ENGINEERING, 2008, 19 : 615 - 622
12345