Structure determination of β-glucans from Ganoderma lucidum with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry

被引：50
|
作者：
Hung, Wei-Ting [1 ]
Wang, Shwu-Huey [2 ]
Chen, Chung-Hsuan [1 ]
Yang, Wen-Bin [1 ]
机构：
[1] Acad Sinica, Gen Res Ctr, Taipei 11529, Taiwan
[2] Taipei Med Univ, Instrument Ctr, Taipei 11031, Taiwan
来源：
MOLECULES | 2008年 / 13卷 / 08期
关键词：
polysaccharides; matrix-assisted laser desorption/ionization (MALDI); Ganoderma lucidum; beta-glucan; 2,5-dihydroxybenzoic acid (2,5-DHB);
D O I：
10.3390/molecules13081538
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, beta-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.
引用
收藏
页码：1538 / 1550
页数：13
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