Measurement of lysophospholipase D/autotaxin activity in human serum samples

被引：50
|
作者：
Nakamura, Kazuhiro
Ohkawa, Ryunosuke
Okubo, Shigeo
Tozuka, Minoru
Okada, Mitsumasa
Aoki, Shinya
Aoki, Junken
Arai, Hiroyuki
Ikeda, Hitoshi
Yatomi, Yutaka
机构：
[1] Univ Tokyo, Dept Clin Lab Med, Grad Sch Med, Bunkyo Ku, Tokyo 1138655, Japan
[2] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Hlth Chem, Tokyo 1138655, Japan
[3] Toho Univ, Dept Biomol Sci, Chiba 2748510, Japan
[4] Tokyo Univ Hosp, Dept Clin Lab, Tokyo 113, Japan
来源：
CLINICAL BIOCHEMISTRY | 2007年 / 40卷 / 3-4期
关键词：
lysophospholipase D; autotaxin; lysophosphatidic acid; lysophosphatidylcholine; plasma;
D O I：
10.1016/j.clinbiochem.2006.10.009
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. Design and methods: LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. Results and conclusions: It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.
引用
收藏
页码：274 / 277
页数：4
相关论文
共 50 条
  • [41] Inhibition of lysophospholipase D activity by fish egg extracts
    Xi-Wen Liu
    Hyun Jung Shim
    Chan Wok Son
    Mi Yeon Kim
    Min Hee Kim
    Hui Song Cui
    Cheon Ho Park
    Dai-Eun Sok
    Mee Ree Kim
    European Food Research and Technology, 2009, 228 : 411 - 416
  • [42] Inhibition of lysophospholipase D activity by fish egg extracts
    Liu, Xi-Wen
    Shim, Hyun Jung
    Son, Chan Wok
    Kim, Mi Yeon
    Kim, Min Hee
    Cui, Hui Song
    Park, Cheon Ho
    Sok, Dai-Eun
    Kim, Mee Ree
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2009, 228 (03) : 411 - 416
  • [43] Measurement of hyaluronidase activity in normal human serum
    Wilkinson, CR
    Bower, LM
    Warren, C
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1996, 14 (06) : 707 - 712
  • [44] Serum Autotaxin Activity Correlates With Pruritus in Pediatric Cholestatic Disorders
    Kremer, Andreas E.
    Gonzales, Emmanuel
    Schaap, Frank G.
    Elferink, Ronald P. J. Oude
    Jacquemin, Emmanuel
    Beuers, Ulrich
    JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 2016, 62 (04): : 530 - 535
  • [45] MEASUREMENT OF VITAMIN D METABOLIC MARKERS IN HUMAN SAMPLES
    Volmer, Dietrich A.
    Mueller, Miriam J.
    Lammert, Frank
    Stokes, Caroline S.
    ANTICANCER RESEARCH, 2015, 35 (06) : 3607 - 3608
  • [46] Serum autotaxin activity correlates with pruritus in paediatric cholestatic diseases
    Kremer, A. E.
    Gonzales, E.
    Schaap, F. G.
    Elferink, R. P. Oude
    Jacquemin, E.
    Beuers, U.
    EXPERIMENTAL DERMATOLOGY, 2014, 23 (03) : E42 - E42
  • [47] Increased formation of lysophosphatidic acids by lysophospholipase D in serum of hypercholesterolemic rabbits
    Tokumura, A
    Kanaya, Y
    Kitahara, M
    Miyake, M
    Yoshioka, Y
    Fukuzawa, K
    JOURNAL OF LIPID RESEARCH, 2002, 43 (02) : 307 - 315
  • [48] Potentials of the Circulating Pruritogenic Mediator Lysophosphatidic Acid in Development of Allergic Skin Inflammation in Mice Role of Blood Cell-Associated Lysophospholipase D Activity of Autotaxin
    Shimizu, Yoshibumi
    Morikawa, Yoshiyuki
    Okudaira, Shinichi
    Kimoto, Shigenobu
    Tanaka, Tamotsu
    Aoki, Junken
    Tokumura, Akira
    AMERICAN JOURNAL OF PATHOLOGY, 2014, 184 (05): : 1593 - 1603
  • [49] Serum autotaxin measurement in haematological malignancies: a promising marker for follicular lymphoma
    Masuda, Akiko
    Nakamura, Kazuhiro
    Izutsu, Koji
    Igarashi, Koji
    Ohkawa, Ryunosuke
    Jona, Masahiro
    Higashi, Katsumi
    Yokota, Hiromitsu
    Okudaira, Shinichi
    Kishimoto, Tatsuya
    Watanabe, Takuro
    Koike, Yukako
    Ikeda, Hitoshi
    Kozai, Yasushi
    Kurokawa, Mineo
    Aoki, Junken
    Yatomi, Yutaka
    BRITISH JOURNAL OF HAEMATOLOGY, 2008, 143 (01) : 60 - 70
  • [50] Specific increase in serum autotaxin activity in patients with pancreatic cancer
    Nakai, Yousuke
    Ikeda, Hitoshi
    Nakamura, Kazuhiro
    Kume, Yukio
    Fujishiro, Mitsuhiro
    Sasahira, Naoki
    Hirano, Kenji
    Isayama, Hiroyuki
    Tada, Minoru
    Kawabe, Takao
    Komatsu, Yutaka
    Omata, Masao
    Aoki, Junken
    Koike, Kazuhiko
    Yatomi, Yutaka
    CLINICAL BIOCHEMISTRY, 2011, 44 (8-9) : 576 - 581
12345