Measurement of lysophospholipase D/autotaxin activity in human serum samples

被引：50

作者：

Nakamura, Kazuhiro

Ohkawa, Ryunosuke

Okubo, Shigeo

Tozuka, Minoru

Okada, Mitsumasa

Aoki, Shinya

Aoki, Junken

Arai, Hiroyuki

Ikeda, Hitoshi

Yatomi, Yutaka

机构：

[1] Univ Tokyo, Dept Clin Lab Med, Grad Sch Med, Bunkyo Ku, Tokyo 1138655, Japan

[2] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Hlth Chem, Tokyo 1138655, Japan

[3] Toho Univ, Dept Biomol Sci, Chiba 2748510, Japan

[4] Tokyo Univ Hosp, Dept Clin Lab, Tokyo 113, Japan

来源：

CLINICAL BIOCHEMISTRY | 2007年 / 40卷 / 3-4期

关键词：

lysophospholipase D; autotaxin; lysophosphatidic acid; lysophosphatidylcholine; plasma;

D O I：

10.1016/j.clinbiochem.2006.10.009

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. Design and methods: LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. Results and conclusions: It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.

引用

页码：274 / 277

页数：4

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