Optimization of cellobiose dehydrogenase production by Schizophyllum commune and effect of the enzyme on kraft pulp bleaching by ligninases

被引：21
|
作者：
Fang, J [1 ]
Huang, F [1 ]
Gao, PJ [1 ]
机构：
[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
来源：
PROCESS BIOCHEMISTRY | 1999年 / 34卷 / 09期
关键词：
cellobiose dehydrogenase; enzyme production; lignin degradation; pulp bleaching;
D O I：
10.1016/S0032-9592(99)00016-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of various parameters on cellobiose dehydrogenase (CDH) production by Schizophyllum commune AS 5.391 were investigated. Among different carbon and nitrogen sources tested, dewaxed cotton powder and diammonium hydrogen phosphate produced the highest titers of CDH. S. commune AS 5.391 produced CDH only when grown on cellulosic substrates but the lignin-related compounds veratryl alcohol and guaiacol had no effect on CDH production. The optimum pH for CDH production was 4.5. Addition of succinate and Tween 80 to the medium significantly improved the enzyme yield. Optimized culture conditions were obtained and the highest level of CDH was 150 U/l. CDH could facilitate kraft pulp lignin degradation by ligninases. The influence of CDH on kraft pulp bleaching by ligninases was also studied. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：957 / 961
页数：5
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