Development and characterization of simple sequence repeat (SSR) markers in the water lotus (Nelumbo nucifera)

被引：35

作者：

Kubo, Nakao ^{[1
]}

Hirai, Masashi ^{[1
]}

Kaneko, Akio ^{[2
]}

Tanaka, Daizo ^{[3
]}

Kasumi, Kumaji ^{[3
]}

机构：

[1] Kyoto Prefectural Univ, Grad Sch Agr, Kyoto Prefectural Inst Agr Biotechnol, Kyoto 6190244, Japan

[2] Kyoto Bot Garden, Sakyo Ku, Shimogamo 6060823, Japan

[3] Kyoto Flower Ctr, Kyoto 6190244, Japan

来源：

AQUATIC BOTANY | 2009年 / 90卷 / 02期

关键词：

DNA marker; Lotus; Microsatellite; Nelumbo; Simple sequence repeat (SSR); MICROSATELLITE MARKERS; GENETIC DIVERSITY; ISSR MARKERS; RAPD; PHYLOGENY; PLANTS;

D O I：

10.1016/j.aquabot.2008.06.006

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Simple sequence repeat (SSR) markers were developed in the water lotus (Nelumbo nucifera Gaertn.) from an SSR-enriched genomic library. Of the SSR markers tested, 11 primer pairs produced clearly distinguishable DNA banding patterns. Forty-three alleles were detected with the 11 markers. The allele number per locus ranged from 2 to 5 with an average of 3.9. Polymorphism values ranged from 0.11 to 0.66 with an average of 0.51. These primers were also applicable to another Nelumbo, species, Nelumbo lutea (Willd.) Pets. (American lotus) and hybrids between N. nucifera and N. lutea. These results indicate that the SSR markers developed in this study are informative and will be useful for genetic analysis in Nelumbo species. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：191 / 194

页数：4

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