Development and use of simple sequence repeat SSR markers in Rubus species

被引：49
|
作者：
Graham, J [1 ]
Smith, K [1 ]
Woodhead, M [1 ]
Russell, J [1 ]
机构：
[1] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland
来源：
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期
关键词：
microsatellite primers; Rubus; red raspberry;
D O I：
10.1046/j.1471-8286.2002.00203.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The isolation of polymorphic codominant microsatellite markers in Rubus and in particular red raspberry will provide a tool to investigate gene flow between cultivated and wild raspberries. Microsatellite loci were isolated by screening a PstI size selected genomic library with AC((13)) and AG((13)). Positive clones were sequenced and primer pairs designed to the sequences flanking identified SSRs. One primer of each pair was fluorescently labelled to facilitate polymerase chain reaction (PCR) product identification on an automated DNA sequencer. We describe 10 polymorphic microsatellite loci developed and demonstrate their usefulness in different Rubus species.
引用
下载
收藏
页码：250 / 252
页数：3
相关论文
共 50 条
  • [1] Development of simple sequence repeat (SSR) markers and their use in identification of Dendrobium varieties
    Yue, G. H.
    Lam-Chan, L. T.
    Hong, Y.
    MOLECULAR ECOLOGY NOTES, 2006, 6 (03): : 832 - 834
  • [2] Simple sequence repeat ssr markers used in rubus species from romanian flora and North-European and North-American rubus cultivars (Rubus idaeus)
    Raluca, Rusu Anda
    Pamfil, D.
    Graham, Julie
    Smith, Kay
    Balteanu, V. A.
    Groza, Gh.
    Bondrea, Ioana
    Patrascu, Bianca
    BULLETIN OF THE UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE, VOL 62, 2006: ANIMAL HUSBANDRY AND BIOTECHNOLOGIES, 2006, 62 : 252 - +
  • [3] Development of simple sequence repeat markers in Cymbopogon species
    Kumar, Jitendra
    Verma, Vijeshwar
    Shahi, Ashok Kumar
    Qazi, Gulam Nab
    Balyan, Harindra Singh
    PLANTA MEDICA, 2007, 73 (03) : 262 - 266
  • [4] Genetic diversity of Dendrobium species revealed by simple sequence repeat (SSR) markers
    Reddy, D. Mahesh
    Momin, Kalkame Ch.
    Singh, Amit Kumar
    Kumar, Sunil
    Wangchu, L.
    Bhargav, V.
    GENETIC RESOURCES AND CROP EVOLUTION, 2024, 71 (04) : 1453 - 1464
  • [5] Genetic diversity of Dendrobium species revealed by simple sequence repeat (SSR) markers
    D. Mahesh Reddy
    Kalkame Ch. Momin
    Amit Kumar Singh
    Sunil Kumar
    L. Wangchu
    V. Bhargav
    Genetic Resources and Crop Evolution, 2024, 71 : 1453 - 1464
  • [6] Genome Survey Sequence and the Development of Simple Sequence Repeat (SSR) Markers inErianthus arundinaceus
    Zeng, Qiaoying
    Huang, Zhenghui
    Wang, Qinnan
    Wu, Jiayun
    Feng, Xiaoming
    Qi, Yongwen
    SUGAR TECH, 2021, 23 (01) : 77 - 85
  • [7] Genome Survey Sequence and the Development of Simple Sequence Repeat (SSR) Markers in Erianthus arundinaceus
    Qiaoying Zeng
    Zhenghui Huang
    Qinnan Wang
    Jiayun Wu
    Xiaoming Feng
    Yongwen Qi
    Sugar Tech, 2021, 23 : 77 - 85
  • [8] Simple Sequence Repeat (SSR) Markers for Raspberry and Blackberry
    Lewers, Kim S.
    Stafne, Eric T.
    Clark, John R.
    Weber, Courtney A.
    Graham, Julie
    HORTSCIENCE, 2004, 39 (04) : 785 - 785
  • [9] Development and characterization of simple sequence repeat (SSR) markers for the mushroom Flammulina velutipes
    Zhang, Ruiying
    Hu, Dandan
    Zhang, Jinxia
    Zuo, Xuemei
    Jiang, Ruibo
    Wang, Hexiang
    Ng, Tzi Bun
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2010, 110 (03) : 273 - 275
  • [10] Transcriptome sequencing of Himalayan Raspberry (Rubus ellipticus) and development of simple sequence repeat markers
    Samriti Sharma
    Rajinder Kaur
    Amol Kumar U. Solanke
    Himanshu Dubey
    Siddharth Tiwari
    Krishan Kumar
    3 Biotech, 2019, 9
12345