Monitoring expression of selected Plasmodiophora brassicae genes during clubroot development in Arabidopsis thaliana

被引：40
|
作者：
Siemens, J. [1 ]
Graf, H. [2 ]
Bulman, S. [3 ]
In, O. [4 ]
Ludwig-Mueller, J. [1 ]
机构：
[1] Tech Univ Dresden, Dept Biol, D-01062 Dresden, Germany
[2] Free Univ Berlin, Inst Genet, D-14195 Berlin, Germany
[3] New Zealand Inst Crop & Food Res, Christchurch, New Zealand
[4] Goethe Univ Frankfurt, Inst Bot, D-60054 Frankfurt, Germany
来源：
PLANT PATHOLOGY | 2009年 / 58卷 / 01期
关键词：
Brassicaceae; clubroot pathogenesis; differential gene expression; root and hypocotyl galls; PATHOGEN; DISEASE; IDENTIFICATION; POLYMORPHISM; CYTOKININS; GROWTH;
D O I：
10.1111/j.1365-3059.2008.01943.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The expression of 12 cDNAs from Plasmodiophora brassicae, among them two novel sequences, was determined during clubroot development on Arabidopsis thaliana. The aim was to find cDNAs expressed at distinct stages of pathogenesis. The relative amount of infection with active plasmodia could be estimated using PbActin cDNA as an internal standard. Two cDNAs, PbBrip9 and PbCC249, were strongly expressed at stages of disease development corresponding to the occurrence of sporulating plasmodia. Therefore, it should be possible in the future to find more cDNAs which could be used as markers for certain stages of clubroot development.
引用
收藏
页码：130 / 136
页数：7
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