Araliaarmoside: A New Triterpene Glycoside Isolated From the Leaves of Aralia armata

One new oleanane-type triterpene glycoside, oleanolic acid-[28-O-beta-d-glucopyranosyl]-3-O-[beta-D-glucopyranosyl(1 -> 6)-beta-D-glucopyranosyl](1 -> 3)[a-l-arabinofuranosyl(1.4)]-beta-D-glucuronopyranoside (1), and 3 known ones {oleanolic acid-[28-O-beta-d-glucopyranosyl]-3-O-[beta-D-galactopyranosyl(1.3)]-[beta ss-D-glucopyranosyl(1.2)]-beta-D-glucuronopyranoside (2) chikusetsusaponin IVa methyl ester (3), and chikusetsusaponin IV (4)} were isolated from the leaves of Aralia armata. Their chemical structures were elucidated using a combination of high-resolution electrospray ionization mass spectrometry, 1-dimensional and 2-dimensional nuclear magnetic resonance (NMR) spectral data, as well as comparison with data in the previous literature. This is the first report of full NMR spectroscopic data of 2. Compounds 1-4 displayed weak cytotoxic activity toward KB and HepG2 cell lines, with half-maximal inhibitory concentration50 values ranging from 24.2 +/- 0.3 to 32.6 +/- 0.8 mu M in in vitro assay.