3D Localization of weak scatterers in digital holographic microscopy using Rayleigh-Sommerfeld back-propagation

被引：47
|
作者：
Wilson, Laurence [1 ]
Zhang, Rongjing [1 ]
机构：
[1] Rowland Inst Harvard, Cambridge, MA 02142 USA
来源：
OPTICS EXPRESS | 2012年 / 20卷 / 15期
关键词：
IN-LINE HOLOGRAPHY; PARTICLE TRACKING; VIDEO MICROSCOPY; LIGHT; RECONSTRUCTION; AMPLITUDE; DYNAMICS; COLLOIDS; SPACE; IMAGE;
D O I：
10.1364/OE.20.016735
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The Rayleigh-Sommerfeld back-propagation method is a fast and highly flexible volume reconstruction scheme for digital holographic microscopy. We present a new method for 3D localization of weakly scattering objects using this technique. A well-known aspect of classical optics (the Gouy phase shift) can be used to discriminate between objects lying on either side of the holographic image plane. This results in an unambiguous, model-free measurement of the axial coordinate of microscopic samples, and is demonstrated both on an individual colloidal sphere, and on a more complex object - a layer of such particles in close contact. (C) 2012 Optical Society of America
引用
收藏
页码：16735 / 16744
页数：10
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