Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum

被引：51
|
作者：
Varallyay, Eva [1 ]
Giczey, Gabor [1 ]
Burgyan, Jozsef [1 ]
机构：
[1] Agr Biotechnol Ctr, Plant Virol Grp, H-2100 Godollo, Hungary
来源：
ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 07期
关键词：
PLANT; BARLEY; WHEAT; DISCOVERY; PROTEIN; DEFENSE; FAMILY;
D O I：
10.1007/s00705-012-1286-y
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Powdery mildew is one of the most important cereal diseases worldwide. Genetic analysis has revealed that mutant alleles of the Mlo gene cause broad-spectrum resistance against this pathogen in barley. In this study, the possibility of inducing broad-spectrum powdery mildew resistance against this pathogen by RNAi of the barley Mlo ortholog in wheat was examined using virus-induced gene silencing (VIGS). A clear correlation was found between resistance and accumulation of Mlo-specific siRNAs, raising the possibility of designing powdery mildew resistance in wheat by RNA silencing using both transgenic and non-transgenic approaches.
引用
收藏
页码：1345 / 1350
页数：6
相关论文
共 50 条
  • [41] Simultaneous silencing of two different Arabidopsis genes with a novel virus-induced gene silencing vector
    Wu, Kunxin
    Wu, Yadan
    Zhang, Chunwei
    Fu, Yan
    Liu, Zhixin
    Zhang, Xiuchun
    PLANT METHODS, 2021, 17 (01)
  • [42] Molecular mapping of powdery mildew resistance gene PmHNK in winter wheat (Triticum aestivum L.) cultivar Zhoumai 22
    W. G. Xu
    C. X. Li
    L. Hu
    L. Zhang
    J. Z. Zhang
    H. B. Dong
    G. S. Wang
    Molecular Breeding, 2010, 26 : 31 - 38
  • [43] Molecular mapping of powdery mildew resistance gene PmHNK in winter wheat (Triticum aestivum L.) cultivar Zhoumai 22
    Xu, W. G.
    Li, C. X.
    Hu, L.
    Zhang, L.
    Zhang, J. Z.
    Dong, H. B.
    Wang, G. S.
    MOLECULAR BREEDING, 2010, 26 (01) : 31 - 38
  • [44] Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.)
    Huang, XQ
    Wang, LX
    Xu, MX
    Röder, MS
    THEORETICAL AND APPLIED GENETICS, 2003, 106 (05) : 858 - 865
  • [45] Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.)
    X. Huang
    L. Wang
    M. Xu
    M. Röder
    Theoretical and Applied Genetics, 2003, 106 : 858 - 865
  • [46] Identification of genes required for the function of non-race-specific mlo resistance to powdery mildew in barley
    Freialdenhoven, A
    Peterhansel, C
    Kurth, J
    Kreuzaler, F
    Schulze-Lefert, P
    PLANT CELL, 1996, 8 (01): : 5 - 14
  • [47] RETRACTED ARTICLE: Virus-induced gene silencing for functional analysis of selected genes
    Mandar R. Godge
    Arunima Purkayastha
    Indranil Dasgupta
    Prakash P. Kumar
    Plant Cell Reports, 2008, 27 : 209 - 219
  • [48] LOCALIZATION OF INDUCED GENES IN BARLEY FOR RESISTANCE AGAINST POWDERY MILDEW
    HEUN, M
    ZEITSCHRIFT FUR PFLANZENZUCHTUNG-JOURNAL OF PLANT BREEDING, 1984, 93 (02): : 158 - 168
  • [49] Virus-induced gene silencing in tomato fruit
    Fu, DQ
    Zhu, BZ
    Zhu, HL
    Jiang, WB
    Luo, YB
    PLANT JOURNAL, 2005, 43 (02): : 299 - 308
  • [50] Virus-induced gene silencing in plant species
    Shao, Y.
    Zhu, H. L.
    Tian, H. Q.
    Wang, X. G.
    Lin, X. J.
    Zhu, B. Z.
    Xie, Y. H.
    Luo, Y. B.
    RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 2008, 55 (02) : 168 - 174
12345