Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum

被引:51
|
作者
Varallyay, Eva [1 ]
Giczey, Gabor [1 ]
Burgyan, Jozsef [1 ]
机构
[1] Agr Biotechnol Ctr, Plant Virol Grp, H-2100 Godollo, Hungary
关键词
PLANT; BARLEY; WHEAT; DISCOVERY; PROTEIN; DEFENSE; FAMILY;
D O I
10.1007/s00705-012-1286-y
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Powdery mildew is one of the most important cereal diseases worldwide. Genetic analysis has revealed that mutant alleles of the Mlo gene cause broad-spectrum resistance against this pathogen in barley. In this study, the possibility of inducing broad-spectrum powdery mildew resistance against this pathogen by RNAi of the barley Mlo ortholog in wheat was examined using virus-induced gene silencing (VIGS). A clear correlation was found between resistance and accumulation of Mlo-specific siRNAs, raising the possibility of designing powdery mildew resistance in wheat by RNA silencing using both transgenic and non-transgenic approaches.
引用
收藏
页码:1345 / 1350
页数:6
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