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Paternity testing of wild black rockfish Sebastes inermis (brownish type) from the Seto Inland Sea of Japan
被引:14
|作者:
Gonzalez, Enrique Blanco
[1
]
Murakami, Tomoya
[2
]
Teshima, Yuki
[1
]
Yoshioka, Kouji
[3
]
Jeong, Dal-Sang
[4
]
Umino, Tetsuya
[1
]
机构:
[1] Hiroshima Univ, Grad Sch Biosphere Sci, Hiroshima 7398528, Japan
[2] Hiroshima Prefectural Technol Res Inst Fisheries, Hiroshima 7371207, Japan
[3] Hiroshima Prefectural Fisheries Coordinat Off, Hiroshima 7308511, Japan
[4] Marine Seed Releasing Technol Ctr, Wando 537806, South Korea
关键词:
Black rockfish;
Sebastes inermis;
Microsatellite DNA;
Mating;
Paternity diagnosis;
MICROSATELLITE DNA MARKERS;
3 COLOR MORPHOTYPES;
MULTIPLE PATERNITY;
SEBASTISCUS-MARMORATUS;
GENETIC BENEFITS;
FISH;
POPULATION;
FEMALES;
SUCCESS;
SYSTEM;
D O I:
10.1007/s10228-008-0055-0
中图分类号:
S9 [水产、渔业];
学科分类号:
0908 ;
摘要:
We developed four microsatellite DNA loci to test for multiple paternity of black rockfish, Sebastes inermis, from the Seto Inland Sea of Japan. All loci showed a high degree of polymorphism (number of alleles per locus = 10-14, expected heterozygosity = 0.80) and discriminating power (probability of identity index = 3.71 x 10(-6), exclusion probability = 0.999) in unrelated wild specimens (n = 32). Genotypic assignment of five dams (109-220 mm in total length) and 50 embryos from each dam (n = 50) indicated that four dams were mated with a single sire. Only for one dam and three of her embryos we could not exclude multiple paternity.
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页码:87 / 91
页数:5
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