Application of an enzyme-linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serovar 15 in pig sera

被引：5

作者：

Teshima, Kaho ^{[1
]}

Lee, Jina ^{[1
]}

To, Ho ^{[1
]}

Kamada, Takashi ^{[1
]}

Tazumi, Akihiro ^{[1
]}

Hirano, Haruna ^{[2
]}

Maruyama, Minoru ^{[3
]}

Ogawa, Torata ^{[4
]}

Nagai, Shinya ^{[1
]}

Turni, Conny ^{[5
]}

Tsutsumi, Nobuyuki ^{[1
]}

机构：

[1] Nippon Inst Biol Sci, 9-2221-1,Shinmachi, Tokyo 1980024, Japan

[2] Yamanashi Prefecture Eastern Livestock Hyg Serv C, 1001-1 Karakashiwa, Fuefuki, Yamanashi 4060034, Japan

[3] Yamanashi Prefecture Livestock Dairy Technol Ctr, 963-1,Otokuro, Chuou, Yamanashi 4093812, Japan

[4] Fukuoka Prefecture Cent Livestock Hyg Serv Ctr, Higashi Ku, 4-14-5,Hakozakihutou, Fukuoka, Fukuoka 8120051, Japan

[5] Univ Queensland, Queensland Alliance Agr & Food Innovat, Ecosci Precinct, Dutton Pk, Qld, Australia

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2017年 / 79卷 / 12期

关键词：

Actinobacillus pleuropneumoniae; ELISA; LPS; pig; serovar; 15; CAPSULAR POLYSACCHARIDE; SUIS; LIPOPOLYSACCHARIDES; SAMPLES;

D O I：

10.1292/jvms.17-0374

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An indirect enzyme-linked immunosorbent assay (ELISA) using lipopolysaccharide extract as antigen was evaluated for detection of antibodies to Actinobacillus pleuropneumoniae serovar 15. The serovar 15 ELISA had a higher sensitivity and specificity than latex agglutination test for 63 and 80 sera from pigs experimentally infected and not infected with A. pleuropneumoniae, respectively. When the serovar 15 ELISA was applied to 454 field sera, high rates of seropositivity were found in pigs from farms infected with A. pleuropneumoniae serovar 15, but not in those from farms free of A. pleuropneumoniae serovar 15. The results suggest that the serovar 15 ELISA may be useful for the serological surveillance of infection with A. pleuropneumoniae serovar 15.

引用

页码：1968 / 1972

页数：5

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