Propagation of orchid Gongora quinquenervis by in vitro germination

被引:10
|
作者
Martini, PC [1 ]
Willadino, L [1 ]
Alves, GD [1 ]
Donato, VMTS [1 ]
机构
[1] UFRPE, BR-52171900 Recife, PE, Brazil
关键词
calli; protocorms; germination; organogenesis;
D O I
10.1590/S0100-204X2001001000015
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The aim of this experiment was to study in vitro germination and organogenesis of Gongora quinquenervis in two culture media (Knudson "C" and Murashige & Skoog) on three concentrations of BAP (0.0, 0.5 and 1.0 mg L-1). The protocorms cultured on Knudson "C" medium died, The majority of the embryos cultured on the Murashige & Skoog medium formed calli. The calli presented a high morfogenetic potential, regenerating great number of plant through indirect organogenesis, and the treatment without BAP presented the highest regeneration frequency Few plants were germinated through the normal route, performing a total of 41 plants, which contrasts with the 715 plants regenerated through indirect organogenesis.
引用
收藏
页码:1319 / 1324
页数:6
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