Juxtamembrane basic residues in glycoprotein Ibβ cytoplasmic domain are required for assembly and surface expression of glycoprotein Ib-IX complex

被引：12
|
作者：
Mo, Xi [1 ]
Luo, Shi-Zhong [1 ]
Lopez, Jose A. [2 ]
Li, Renhao [1 ]
机构：
[1] Univ Texas Hlth Sci Ctr Houston, Ctr Membrane Biol, Dept Biochem & Mol Biol, Houston, TX 77030 USA
[2] Univ Washington, Dept Med, Puget Sound Blood Ctr, Div Hematol, Seattle, WA 98104 USA
来源：
FEBS LETTERS | 2008年 / 582卷 / 23-24期
基金：
美国国家卫生研究院;
关键词：
GPIb-IX complex; Juxtamembrane region; Polybasic residue cluster; Protein biosynthesis; Trafficking;
D O I：
10.1016/j.febslet.2008.08.036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Platelet glycoprotein (GP) Ib-IX complex requires all its three subunits for efficient expression on the cell surface, but the underlying molecular basis is not fully clear. Using transfected Chinese hamster ovary cells as the model system, we demonstrate that juxtamembrane residues 149-154 in the cytoplasmic domain of the GPIb beta subunit is required for assembly and surface expression of the GPIb-IX complex. The complex, or GPIbb by itself, lacking these residues is retained in the endoplasmic reticulum. Our results thus have illustrated an important role of the GPIbb cytoplasmic domain in biosynthesis of the GPIb-IX complex.
引用
收藏
页码：3270 / 3274
页数：5
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