Progresses of CRISPR/Cas9 genome editing in forage crops

被引:6
|
作者
Haq, Syed Inzimam Ul [1 ]
Zheng, Dianfeng [4 ]
Feng, Naijie [4 ]
Jiang, Xingyu [4 ]
Qiao, Feng [3 ]
He, Jin-Sheng [2 ]
Qiu, Quan-Sheng [1 ,2 ,3 ,4 ,5 ]
机构
[1] Lanzhou Univ, Sch Life Sci, MOE Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China
[2] Lanzhou Univ, State Key Lab Herbage Improvement & Grassland Agro, Lanzhou 730000, Gansu, Peoples R China
[3] Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Sch Life Sci, Xining 810016, Qinghai, Peoples R China
[4] Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Guangdong, Peoples R China
[5] Lanzhou Univ, Sch Life Sci, Lanzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
CRISPR; Cas9; Genome editing; Breeding; Forages; DOUBLE-STRAND BREAKS; TARGETED MUTAGENESIS; MEDICAGO-TRUNCATULA; HOMOLOGOUS RECOMBINATION; DNA-BINDING; CHLOROPHYLL DEGRADATION; GIBBERELLIN METABOLISM; PROPOSED NOMENCLATURE; FLOWER DEVELOPMENT; APICAL DOMINANCE;
D O I
10.1016/j.jplph.2022.153860
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) mediated-genome editing has evolved into a powerful tool that is widely used in plant species to induce editing in the genome for analyzing gene function and crop improvement. CRISPR/Cas9 is an RNA-guided genome editing tool consisting of a Cas9 nuclease and a single-guide RNA (sgRNA). The CRISPR/Cas9 system enables more accurate and efficient genome editing in crops. In this review, we summarized the advances of the CRISPR/Cas9 technology in plant genome editing and its applications in forage crops. We described briefly about the development of CRISPR/Cas9 technology in plant genome editing. We assessed the progress of CRISPR/Cas9-mediated targeted-mutagenesis in various forage crops, including alfalfa, Medicago truncatula, Hordeum vulgare, Sorghum bicolor, Setaria italica and Panicum virgatum. The potentials and challenges of CRISPR/Cas9 in forage breeding were discussed.
引用
收藏
页数:14
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