Field-of-View Correction for Dual-Camera Dynamic Phase Imaging Based on Transport of Intensity Equation

In microscopic imaging, dual-camera dynamic phase imaging based on the transport of intensity equation (TIE) is an effective method for the quantitative observation of phase imaging of living cells. However, camera installation errors cause the difference in field-of-views of two cameras. Consequently, the phase obtained using TIE is inaccurate. This study proposes a correction method based on checkerboard calibration to eliminate the mismatch with the sub-pixel aligning accuracy. The feasibility and accuracy of the proposed method arc verified by quantitatively imaging measurement for a standard microlens array. The dynamic phase imaging of the living Haematococcus pluvialis cells is successfully realized, indicating that the method has the potential for wide applications in the field of dynamic bio-microscopy imaging.