Determination of the complex refractive index of highly concentrated hemoglobin solutions using transmittance and reflectance measurements

被引：91
|
作者：
Friebel, M
Meinke, M
机构：
[1] Charite Univ Med Berlin, Inst Med Phys & Lasermed, D-14195 Berlin, Germany
[2] Laser & Med Technol GmbH, D-14195 Berlin, Germany
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2005年 / 10卷 / 06期
关键词：
complex refractive index; hemoglobin; transmittance; Fresnel reflectance;
D O I：
10.1117/1.2138027
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The complex refractive index of highly concentrated hemoglobin solutions as they appear in red blood cells are determined in the wavelength range of 250 to 1100 nm using transmittance and Fresnel reflectance measurements. The determined real parts of the refractive indices are on average 0.02 units higher than the values found in the literature. The wavelength dependence of the measured data in the UV region differs from the calculated data using the Kramers-Kronig relation. (C) 2005 Society of Photo-Optical Instrumentation Engineers.
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页数：5
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