Histopathological aspects of skin in venous leg ulceration caused by chronical venous insufficiency

Prolonged venous hypertension change both the conditions of local skin microcirculation and selecting of fibriloformative cells, resulting in appearance and evolution of venous leg ulceration. During 2003-2004, investigations were completed on 76 biopsies speciments from 27 pacients with venous ulceration caused by CVI of lower limbs, the class 4 (n = 12), class 5 (n = 6) and class 6 (n = 9) after CEAP classification. The biopsies speciments were serial excised from the ulceration basis, ulceration edges, the periulceration area of 2 cm. The biopsies specimens were processed applying a usual histological technique, histochemistry technique (orcein, impregnation silver, blue alcian). Also were immunohistochemistry investigated 5 cases in LSAB(2) technique. In adjacent dermal of ulceration lesions were observed pronounced fibrosis, unspecifical chronic inflammation, high neoangiogenesis in deep and superficial dermis and in varicose veins walls, dots-like distribution of dermal myofibroblasts.