Induction of stable RNA interference in mammalian cells

被引：56

作者：

Cullen, BR

机构：

[1] Duke Univ, Med Ctr, Ctr Virol, Durham, NC 27710 USA

[2] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA

来源：

GENE THERAPY | 2006年 / 13卷 / 06期

关键词：

RNA interference; micro-RNAs; short hairpin RNAs; lentiviral vectors;

D O I：

10.1038/sj.gt.3302656

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, experimental steps and expression vectors that can facilitate the effective knockdown of almost any vertebrate gene product in cultured cells or in experimental animals.

引用

页码：503 / 508

页数：6

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