A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae

被引：22

作者：

Fan, Ligang ^{[1
,2
]}

Wang, Tingting ^{[2
]}

Hua, Canfeng ^{[2
]}

Sun, Wenju ^{[1
]}

Li, Xiaoyu ^{[1
]}

Grunwald, Lucas ^{[2
]}

Liu, Jingui ^{[2
]}

Wu, Nan ^{[1
]}

Shao, Xiaolong ^{[2
]}

Yin, Yimeng ^{[3
]}

Yan, Jian ^{[1
,2
]}

Deng, Xin ^{[2
]}

机构：

[1] Northwest Univ, Sch Med, Xian 710069, Peoples R China

[2] City Univ Hong Kong, Dept Biomed Sci, Kowloon Tong, Hong Kong, Peoples R China

[3] Tongji Univ, Sch Med, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Like Intelligence, Shanghai 200081, Peoples R China

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

PV. TOMATO DC3000; III SECRETION SYSTEM; 2-COMPONENT SYSTEM; RESPONSE REGULATOR; PROTEIN SECRETION; GENOME SEQUENCE; PATHOGEN; GENES; EXPRESSION; VIRULENCE;

D O I：

10.1038/s41467-020-18744-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.

引用

页数：11

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