Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags

被引：20
|
作者：
Ng, SHS
Chang, A
Brown, GD
Koop, BF
Davidson, WS [1 ]
机构：
[1] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada
[2] Univ Victoria, Ctr Biomed Res, Dept Biol, Victoria, BC V8P 5C2, Canada
来源：
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期
关键词：
Atlantic salmon; ESTs; microsatellites; type I markers;
D O I：
10.1111/j.1471-8286.2005.01056.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST database. We designed primers to amplify 158 SSRs, of which 65 amplified 76 loci (including 11 duplicated loci). Sixty-one of the 76 loci were variable in 24 Atlantic salmon from seven populations, and 96% of these markers also amplify DNA from other salmonids. Functions for 16 of the SSR associated ESTs have been determined, confirming them as Type I markers.
引用
收藏
页码：762 / 766
页数：5
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