Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7

被引:23
|
作者
Sigida, Elena N. [1 ]
Fedonenko, Yuliya P. [1 ]
Shashkov, Alexander S. [2 ]
Zdorovenko, Evelina L. [2 ]
Konnova, Svetlana A. [1 ,3 ]
Ignatov, Vladimir V. [1 ,3 ]
Knirel, Yuriy A. [2 ]
机构
[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia
[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[3] Saratov NG Chernyshevskii State Univ, Saratov 410012, Russia
基金
俄罗斯基础研究基金会;
关键词
Lipopolysaccharide; Bacterial polysaccharide structure; Azospirillum brasilense; POLAR FLAGELLUM; WHEAT; MONOSACCHARIDES; CLASSIFICATION; RHIZOSPHERE; CHAIN; GENUS;
D O I
10.1016/j.carres.2013.07.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipopolysaccharide was obtained by phenol-water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and H-1 and C-13 NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (similar to 40%) 2-O-methylation of L-rhamnose. [GRAPHICS] (C) 2013 Elsevier Ltd. All rights reserved.
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页码:76 / 80
页数:5
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