Japanese Encephalitis DNA Vaccines with Epitope Modification Reduce the Induction of Cross-Reactive Antibodies against Dengue Virus and Antibody-Dependent Enhancement of Dengue Virus Infection

被引:4
|
作者
Kotaki, Tomohiro [1 ,2 ]
Nagai, Yurie [1 ]
Yamanaka, Atsushi [3 ,4 ]
Konishi, Eiji [3 ]
Kameoka, Masanori [1 ]
机构
[1] Kobe Univ, Dept Publ Hlth, Grad Sch Hlth Sci, Kobe, Hyogo 6540142, Japan
[2] Osaka Univ, Res Inst Microbial Dis, Dept Virol, Suita, Osaka 5650871, Japan
[3] Mahidol Univ, Fac Trop Med, BIKEN Endowed Dept Dengue Vaccine Dev, Bangkok 10400, Thailand
[4] Mahidol Univ, Fac Trop Med, Mahidol Osaka Ctr Infect Dis, Bangkok 10400, Thailand
关键词
Japanese encephalitis virus; dengue virus; vaccine; antibody-dependent enhancement; NEUTRALIZING ANTIBODIES; IMMUNIZATION; SURVEILLANCE; PROTECT; TYPE-1; ASSAY;
D O I
10.3390/vaccines10091411
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Infection with viruses belonging to the genus Flavivirus, such as Japanese encephalitis virus (JEV) and dengue virus (DENV), is a worldwide health problem. Vaccines against JEV and DENV are currently available. However, the dengue vaccine possibly increases the risk of severe dengue due to antibody-dependent enhancement (ADE). Moreover, the Japanese encephalitis (JE) vaccine reportedly induces cross-reactive ADE-prone antibodies against DENV, potentially leading to symptomatic dengue. Therefore, it is necessary to eliminate the risk of ADE through vaccination. In this study, we attempted to develop a JE vaccine that does not induce ADE of DENV infection using an epitope modification strategy. We found that an ADE-prone monoclonal antibody cross-reactive to DENV and JEV recognizes the 106th amino acid residue of the E protein of JEV (E-106). The JE DNA vaccine with a mutation at E-106 (E-106 vaccine) induced comparable neutralizing antibody titers against JEV to those induced by the wild-type JE DNA vaccine. Meanwhile, the E-106 vaccine induced 64-fold less cross-reactive ADE-prone antibodies against DENV. The mutation did not compromise the protective efficacy of the vaccine in the lethal JEV challenge experiment. Altogether, the modification of a single amino acid residue identified in this study helped in the development of an ADE-free JE vaccine.
引用
收藏
页数:16
相关论文
共 50 条
  • [1] The Complexity of Antibody-Dependent Enhancement of Dengue Virus Infection
    Guzman, Maria G.
    Vazquez, Susana
    [J]. VIRUSES-BASEL, 2010, 2 (12): : 2649 - 2662
  • [2] Structural perspectives of antibody-dependent enhancement of infection of dengue virus
    Morrone, Seamus R.
    Lok, Shee-Mei
    [J]. CURRENT OPINION IN VIROLOGY, 2019, 36 : 1 - 8
  • [3] Dengue virus cross-reactive hemagglutination inhibition antibody responses in patients with primary dengue virus infection
    Anantapreecha, Surapee
    A-nuegoonpipat, Atchareeya
    Prakrong, Songthum
    Chanama, Sumalee
    Sa-ngasang, Areerat
    Sawanpanyalert, Pathom
    Kurane, Ichiro
    [J]. JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2007, 60 (05) : 267 - 270
  • [4] Antibody-dependent enhancement in dengue virus infections
    Burke, DS
    Kliks, S
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2006, 193 (04): : 601 - 603
  • [5] Dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus
    Dejnirattisai, Wanwisa
    Supasa, Piyada
    Wongwiwat, Wiyada
    Rouvinski, Alexander
    Barba-Spaeth, Giovanna
    Duangchinda, Thaneeya
    Sakuntabhai, Anavaj
    Cao-Lormeau, Van-Mai
    Malasit, Prida
    Rey, Felix A.
    Mongkolsapaya, Juthathip
    Screaton, Gavin R.
    [J]. NATURE IMMUNOLOGY, 2016, 17 (09) : 1102 - 1108
  • [6] Dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus
    Wanwisa Dejnirattisai
    Piyada Supasa
    Wiyada Wongwiwat
    Alexander Rouvinski
    Giovanna Barba-Spaeth
    Thaneeya Duangchinda
    Anavaj Sakuntabhai
    Van-Mai Cao-Lormeau
    Prida Malasit
    Felix A Rey
    Juthathip Mongkolsapaya
    Gavin R Screaton
    [J]. Nature Immunology, 2016, 17 : 1102 - 1108
  • [7] IFITM Proteins Restrict Antibody-Dependent Enhancement of Dengue Virus Infection
    Chan, Ying Kai
    Huang, I-Chueh
    Farzan, Michael
    [J]. PLOS ONE, 2012, 7 (03):
  • [8] The dual-specific binding of dengue virus and target cells for the antibody-dependent enhancement of dengue virus infection
    Huang, Kao-Jean
    Yang, Yu-Ching
    Lin, Yee-Shin
    Huang, Jyh-Hsiung
    Liu, Hsiao-Sheng
    Yeh, Trai-Ming
    Chen, Shun-Hua
    Liu, Ching-Chuan
    Lei, Huan-Yao
    [J]. JOURNAL OF IMMUNOLOGY, 2006, 176 (05): : 2825 - 2832
  • [9] Role of dendritic cells in antibody-dependent enhancement of dengue virus infection
    Boonnak, Kobporn
    Slike, Bonnie M.
    Burgess, Timothy H.
    Mason, Randall M.
    Wu, Shuenn-Jue
    Sun, Peifang
    Porter, Kevin
    Rudiman, Irani Fianza
    Yuwono, Djoko
    Puthavathana, Pilaipan
    Marovich, Mary A.
    [J]. JOURNAL OF VIROLOGY, 2008, 82 (08) : 3939 - 3951
  • [10] MEASUREMENT OF ANTIBODY-DEPENDENT INFECTION ENHANCEMENT OF 4 DENGUE VIRUS SEROTYPES BY MONOCLONAL AND POLYCLONAL ANTIBODIES
    MORENS, DM
    HALSTEAD, SB
    [J]. JOURNAL OF GENERAL VIROLOGY, 1990, 71 : 2909 - 2914