Expression of HIV-1 Vif and Human APOBEC3G with a Bicistronic Vector

被引：0
|
作者：
Yang Yishu [1 ]
Sun Xiaona [1 ]
Liu Di [1 ]
Wang Xiaoli [1 ]
Shen Sisi [1 ]
Li Zelin [1 ]
Zeng Yi [1 ]
机构：
[1] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing, Peoples R China
来源：
MECHANICAL ENGINEERING AND MATERIALS SCIENCE | 2012年 / 108卷
关键词：
Human immunodeficiency virus (HIV); apolipoprotein b mRNA editing enzyme-catalytic polypeptide-like 3G (APOBEC3G); viral infectivity factor (Vif); eukaryotic bicistronic vector; ANTIVIRAL ACTIVITY; CYTIDINE DEAMINASE; DNA; PROTEIN; DEGRADATION; HYPERMUTATION; INFECTIVITY; CEM15;
D O I：
10.4028/www.scientific.net/AMM.108.141
中图分类号：
TH [机械、仪表工业];
学科分类号：
0802 ;
摘要：
Novel targets against HIV-1 are booming recently. APOBEC3G has the potential to inhibit the replication of HIV-1, while its antiviral activity is counteracted by Vif. The antiviral mechanism of APOBEC3G and the antagonistic mechanism of Vif have drawn great attention. Considering the occurrence of interaction between Vif and APOBEC3G should be based on that these two proteins coexist in the same cells, a eukaryotic bicistronic vector was adopted to express both Vif and APOBEC3G simultaneously. The expression and distribution of Vif and APOBEC3G proteins are detected with fluorescent microscope.
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页码：141 / 145
页数：5
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