Expression of HIV-1 Vif and Human APOBEC3G with a Bicistronic Vector

被引：0
|
作者：
Yang Yishu [1 ]
Sun Xiaona [1 ]
Liu Di [1 ]
Wang Xiaoli [1 ]
Shen Sisi [1 ]
Li Zelin [1 ]
Zeng Yi [1 ]
机构：
[1] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing, Peoples R China
来源：
MECHANICAL ENGINEERING AND MATERIALS SCIENCE | 2012年 / 108卷
关键词：
Human immunodeficiency virus (HIV); apolipoprotein b mRNA editing enzyme-catalytic polypeptide-like 3G (APOBEC3G); viral infectivity factor (Vif); eukaryotic bicistronic vector; ANTIVIRAL ACTIVITY; CYTIDINE DEAMINASE; DNA; PROTEIN; DEGRADATION; HYPERMUTATION; INFECTIVITY; CEM15;
D O I：
10.4028/www.scientific.net/AMM.108.141
中图分类号：
TH [机械、仪表工业];
学科分类号：
0802 ;
摘要：
Novel targets against HIV-1 are booming recently. APOBEC3G has the potential to inhibit the replication of HIV-1, while its antiviral activity is counteracted by Vif. The antiviral mechanism of APOBEC3G and the antagonistic mechanism of Vif have drawn great attention. Considering the occurrence of interaction between Vif and APOBEC3G should be based on that these two proteins coexist in the same cells, a eukaryotic bicistronic vector was adopted to express both Vif and APOBEC3G simultaneously. The expression and distribution of Vif and APOBEC3G proteins are detected with fluorescent microscope.
引用
收藏
页码：141 / 145
页数：5
相关论文
共 50 条
  • [1] The structural basis for HIV-1 Vif antagonism of human APOBEC3G
    Li, Yen-Li
    Langley, Caroline A.
    Azumaya, Caleigh M.
    Echeverria, Ignacia
    Chesarino, Nicholas M.
    Emerman, Michael
    Cheng, Yifan
    Gross, John D.
    [J]. NATURE, 2023, 615 (7953) : 728 - +
  • [2] The structural basis for HIV-1 Vif antagonism of human APOBEC3G
    Yen-Li Li
    Caroline A. Langley
    Caleigh M. Azumaya
    Ignacia Echeverria
    Nicholas M. Chesarino
    Michael Emerman
    Yifan Cheng
    John D. Gross
    [J]. Nature, 2023, 615 : 728 - 733
  • [3] Identification of the HIV-1 Vif and Human APOBEC3G Protein Interface
    Letko, Michael
    Booiman, Thijs
    Kootstra, Neeltje
    Simon, Viviana
    Ooms, Marcel
    [J]. CELL REPORTS, 2015, 13 (09): : 1789 - 1799
  • [4] The HIV-1 Vif PPLP motif is necessary for human APOBEC3G binding and degradation
    Donahue, John P.
    Vetter, Michael L.
    Mukhtar, Nizar A.
    D'Aquila, Richard T.
    [J]. VIROLOGY, 2008, 377 (01) : 49 - 53
  • [5] Polyubiquitination of APOBEC3G Is Essential for Its Degradation by HIV-1 Vif
    Shao, Qiujia
    Wang, Yudi
    Hildreth, James E. K.
    Liu, Bindong
    [J]. JOURNAL OF VIROLOGY, 2010, 84 (09) : 4840 - 4844
  • [6] HIV-1 Vif and APOBEC3G: Multiple roads to one goal
    Goncalves J.
    Santa-Marta M.
    [J]. Retrovirology, 1 (1)
  • [7] Simian Immunodeficiency Virus Vif and Human APOBEC3B Interactions Resemble Those between HIV-1 Vif and Human APOBEC3G
    Wang, Jiayi
    Shaban, Nadine M.
    Land, Allison M.
    Brown, William L.
    Harris, Reuben S.
    [J]. JOURNAL OF VIROLOGY, 2018, 92 (12)
  • [8] In vitro expression and molecular level analysis of HIV-1 vif and APOBEC3G/B/F
    Zhang, Y. -Z.
    Jiang, X. -Y.
    Wang, Z. -Y.
    Chen, H.
    Lu, H. -Z.
    [J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2010, 14 : S3 - S3
  • [9] Reassessing APOBEC3G Inhibition by HIV-1 Vif-Derived Peptides
    Richards, Christopher M.
    Li, Ming
    Perkins, Angela L.
    Rathore, Anurag
    Harki, Daniel A.
    Harris, Reuben S.
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2017, 429 (01) : 88 - 96
  • [10] APOBEC3G Impairs the Multimerization of the HIV-1 Vif Protein in Living Cells
    Batisse, Julien
    Guerrero, Santiago Xavier
    Bernacchi, Serena
    Richert, Ludovic
    Godet, Julien
    Goldschmidt, Valerie
    Mely, Yves
    Marquet, Roland
    de Rocquigny, Hugues
    Paillart, Jean-Christophe
    [J]. JOURNAL OF VIROLOGY, 2013, 87 (11) : 6492 - 6506
12345