A new chemical method of synthesis of modified nucleoside [32p] phosphates

被引：0

作者：

Yanvarev, D. V. ^{[1
]}

Shirokova, E. A. ^{[1
]}

Kukhanova, M. K. ^{[1
]}

Skoblov, Yu. S. ^{[2
]}

机构：

[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia

[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgon Chem, Moscow 177997, Russia

来源：

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 2008年 / 51卷 / 7-8期

关键词：

modified nucleosides; P-32]orthophosphoric acid; phosphorylation; synthesis of [P-32] labeled nucleotides;

D O I：

10.1002/jlcr.1522

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method of chemical phosphorylation for modified nucleosides with [P-32]orthophosphoric acid in the presence of BrCN is described. The yields of 5'-[P-32]nucleoside monophosphates achieved are 50-65% at nominal specific radioactivities of ca. 1000 Ci/mmol. The mechanism of phosphorylation in the presence of heterocyclic amines is studied.

引用

页码：303 / 307

页数：5

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