A new chemical method of synthesis of modified nucleoside [32p] phosphates

被引：0
|
作者：
Yanvarev, D. V. [1 ]
Shirokova, E. A. [1 ]
Kukhanova, M. K. [1 ]
Skoblov, Yu. S. [2 ]
机构：
[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia
[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgon Chem, Moscow 177997, Russia
来源：
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 2008年 / 51卷 / 7-8期
关键词：
modified nucleosides; P-32]orthophosphoric acid; phosphorylation; synthesis of [P-32] labeled nucleotides;
D O I：
10.1002/jlcr.1522
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple method of chemical phosphorylation for modified nucleosides with [P-32]orthophosphoric acid in the presence of BrCN is described. The yields of 5'-[P-32]nucleoside monophosphates achieved are 50-65% at nominal specific radioactivities of ca. 1000 Ci/mmol. The mechanism of phosphorylation in the presence of heterocyclic amines is studied.
引用
收藏
页码：303 / 307
页数：5
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