Invasive pulmonary aspergillosis diagnosed early by polymerase chain reaction assay

被引：8
|
作者：
Kawamura, S [1 ]
Maesaki, S [1 ]
Omagari, K [1 ]
Hashiguchi, K [1 ]
Tomono, K [1 ]
Tashiro, T [1 ]
Kohno, S [1 ]
机构：
[1] Nagasaki Univ, Sch Med, Dept Internal Med 2, Nagasaki 8528501, Japan
来源：
INTERNAL MEDICINE | 1999年 / 38卷 / 09期
关键词：
serodiagnosis; Aspergillus fumigatus; enzyme-linked immunosorbent assay (ELISA); galactomannan; prednizolone;
D O I：
10.2169/internalmedicine.38.744
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
We compared the usefulness of a polymerase chain reaction (PCR) assay for the early diagnosis of invasive pulmonary aspergillosis with the serodiagnosis of sufficient concentrations of galactomannan using the same serum samples. A patient was treated with prednisolone for the management of hepatitis. Computed tomography (CT) scan of the chest showed the nodular shadow with a cavity containing a clear fungus ball. DNA of Aspergillus spp. from a serum sample was detected and using the same serum sample, both latex agglutination and sandwich enzyme-linked immunosorbent assay (ELISA) of galactomannan were negative. PCR assay provides an early diagnosis of invasive pulmonary aspergillosis compared with ELISA of galactomannan.
引用
收藏
页码：744 / 746
页数：3
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