Long- and short-term effects of androgens in human umbilical artery smooth muscle

被引：22
|
作者：
Saldanha, Paulo A. [1 ]
Cairrao, Elisa [1 ]
Maia, Claudio J. [1 ]
Verde, Ignacio [1 ]
机构：
[1] Univ Beira Interior, Hlth Sci Res Ctr, P-6200506 Covilha, Portugal
来源：
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 2013年 / 40卷 / 03期
关键词：
androgens; calcium channels; potassium channels; smooth muscle cell; vasorelaxation; TESTOSTERONE-INDUCED RELAXATION; K+ CHANNELS; CORONARY-ARTERIES; LARGE-CONDUCTANCE; INDUCED VASORELAXATION; POTASSIUM CHANNELS; CALCIUM-CHANNELS; EXPRESSION; BETA-1-SUBUNIT; ENDOTHELIUM;
D O I：
10.1111/1440-1681.12047
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The aim of the present study was to determine the effects of androgens in the regulation of human umbilical artery (HUA) contractility. The short-term effects of testosterone on the tone of the HUA were investigated, as were the long-term effects of dihydrotestosterone (DHT) on the expression of some proteins involved in the contractile process. Endothelium-denuded HUA were treated for 24h with DHT (2mol/L) or the vehicle control (ethanol) to analyse the genomic effects of androgens. Twenty-four hour treatment of HUA with DHT increased the mRNA expression of the 1-subunit of the large-conductance Ca2+-activated (BKCa) channel and decreased expression of the -subunit of L-type calcium channels. In organ bath studies, testosterone (1100mol/L) produced similar relaxant responses in DHT- and vehicle-treated HUA rings precontracted with 5-HT, histamine and KCl. However, the relaxation response obtained by the combined application of testosterone (100mol/L) and nifedipine (10mol/L) was significantly greater in DHT- compared with vehicle-treated HUA. The results indicate that the rapid vasorelaxant effects of testosterone that are dependent on both BKCa and voltage-sensitive potassium (KV) channel activity in control arteries become dependent solely on KV channel activity in DHT-treated HUA. Thus, the present study reveals the importance of the investigation of both the short- and long-term effects of androgens in human arteries.
引用
收藏
页码：181 / 189
页数：9
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