Analysis of polysaccharides by ultracentrifugation. Size, conformation and interactions in solution

被引：24
|
作者：
Harding, SE [1 ]
机构：
[1] Univ Nottingham, Sch Biosci, NCMH Phys Biochem Lab, Loughborough LE12 5RD, Leics, England
来源：
POLYSACCHARIDES 1: STRUCTURE, CHARACTERIZATION AND USE | 2005年 / 186卷
关键词：
complex; flexibility; molecular weight distribution; mucoadhesion; sedimentation coefficient distribution;
D O I：
10.1007/b136821
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The launch of the XL-I analytical ultracentrifuge by Beckman Instruments (Palo Alto, USA) in 1996 and subsequent development of penetrating software for the analysis of the optical records digitally recorded in this new generation instrument has made some exciting possibilities for the analysis of polysaccharides in a solution environment. We review these developments and investigate the application of the technique to the study of polysaccharide polydispersity, molecular weight analysis, conformation and flexibility analysis, the study of associative interactions, including large complex formation phenomena, and to the measurement of charge and charge-shielding phenomena.
引用
收藏
页码：211 / 254
页数：44
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